Ginger Bioactive Compunds increased Intracellular Antioksidant in Vitro

Tejasari dan F.R. Zakaria (FTP Univ. Jember, Dept. Pangan dan Gizi. IPB)

Abstract

Glutathione (γ-glutamil-sisteinil-glisin) is non protein thiol compound abundance in animal tissue and eucaryote cells, including lymphocyte. It plays important roles in many cellular functions, such as DNA and protein synthesis, xenobiotic-carcinogenic substance detoxification, and immune function maintenance. This  experiment was designed for investigating the effect of ginger bioactive compounds i.o oleoresin, gingerol, and shogaol on glutathione in lymphocyte cultured in normal and under stress oxidative conditions, respectively. Using spectrofotometry measurement, it was known that lymphocyte used in this study had 0,43 μg glutathione /103 cell. Effect of ginger oleoresin, gingerol, and shogaol on lymphocyte’s glutathione content was depend greatly on culture conditions. In culture without stress oxidation, 50 μg/ml treatment of ginger oleoresin, gingerol, and shogaol significantly increased lymphocyte’s glutathione content, respectively, by increament of 449, 188 and 172 percent. The response curve of oleoresin and shogaol was quadratic, while gingerol’s was cubic. Similarly, under stress oxidation condition, oleoresin (200 μg/ml), gingerol (100 μg/ml), and shogaol (200 μg/ml) compounds increased lymphocyte’s glutathion concentration significantly by increament of 563, 1553, and 531 percent, respectively. The response curve of oleoresin was cubic, while gingerol’s and shogaol’s was quadratic. The effect of oleoresin, gingerol, and shogaol on the increament of glutathione content of lymphocyte cultured under stress oxidative was higher than lymphochite cultured without stree oxidative.

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