KONDISI OPTIMUM ENZIM LIPASE KASAR DARI KENTOS KELAPA

(Optimum Conditions Of Rough Lipase Enzym from Coconut Houstorium )

             Moh. Su’i [1]), Harijono 2), Yunianta 2), Aulani’am 3)

[1]) Dosen Teknologi Hasil Pertanian Universitas Widyagama Malang

2) Dosen Teknologi Pertanian Universitas Brawijaya Malang

3) Dosen FMIPA Universitas Brawijaya Malang

 

Abstract

This research learn about  optimum condition (temperature, pH and incubation time). Lipase isolated from coconut houstorium. Houstorium was taken from coconut that has been grew for 30 days in the darkplace and rate temperature. Determination of optimum conditionswas done by examination the  lipase activity in variation condition of temperature (30 – 80 oC), pH (6 – 8,5) and  incubation time (30 – 210 minutes). The results showed that optimum condition of lipase isolated from coconut houstorium were 60 oC, pH 7 and 90 minutes.

Abstrak

Penelitian ini mempelajari kondisi optimum (suhu, pH dan lama inkubasi)  lipase dari kentos buah kelapa. Kentos diperoleh dari kelapa yang telah ditunaskan selama 30 hari di tempat gelap pada suhu ruang. Penentuan kondisi optimum dilakukan dengan menguji aktivitas enzim lipase pada variasi suhu inkubasi (30 – 80 oC). kemudian variasi   pH mulai 6 – 8,5 dan lama inkubasi  antara 30 – 210 menit.  Hasil penelitian menunjukkan bahwa kondisi optimum terdapat pada suhu 60 oC, pH 7 dan lama inkubasi 90 menit.

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